Creatine and creatine kinase measurement.
نویسندگان
چکیده
*Many methods for measuring creatine are known, the principal ones being direct calorimetric assay (l), indirect calorimetric assay (2), and destruction with bacterial adaptive enzymes (3). However, none of these methods are specific nor are they sensitive enough to detect very small amounts of creatine. rIhese assays have been used to examine urine and serum for creatine content, but, because of their liiitations, there is little agreement ahout creatine concentrations in these fluids. It was felt that any study involving creatine would be considerably facilitated by an improved means of determination. In recent years, enzymatic assays have been increasingly used for the measurement of biological materials (4, 5). This report describes an enzymatic assay for the determinaton of creatine and its application to creatine measurement in serum and urine.
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ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 234 شماره
صفحات -
تاریخ انتشار 1959